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The endcapped cyanopropyl phase is based on high purity NUCLEODUR^® silica gel. This HPLC phase is able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications. The polarity can be classified as intermediate based on multiple retention mechanisms such as dipole-dipole, π-π, and also hydrophobic interactions. Therefore, this phase shows a distinct selectivity for polar organic compounds as well as for molecules containing π electron systems (e.g., analytes with double bonds). Cyanopropyl HPLC columns can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase.