For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.

ROTI^®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI^®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.

Hot start version of the recombinant heat stable Taq DNA polymerase from the thermophilic bacterium Thermus aquaticus in storage buffer, plus additional 10x concentrated PCR reaction buffer and 10x concentrated PCR reaction buffer with red gel loading dye. ROTI^®Pol Hot-TaqS is recommended for use in highly specific PCR applications. This polymerase set ROTI^®Pol Hot-TaqS is outstandingly suitable for all Taq-based cycling protocols, in which particularly specific amplification is the main focus - as being performed in, for instance, prior to cloning or sequencing processes, in cycle sequencing, and in similar assays. In combination with our unique buffers, the Hot-TaqS polymerase delivers highly specific PCR amplification of good yield with a wide range of PCR templates. The antibody-mediated blocking of the DNA polymerase is released only at the initial denaturation step, hence resulting in highly specific amplification of the target sequence without production of unwanted side products caused by unspecific primer annealing.ROTI^®Pol Hot-TaqS is able to amplify PCR products up to 3 kb with genomic DNA and up to at least 5 kb in size with Lambda DNA and is appropriate for use in the amplification of DNA from genomic, viral, and plasmid templates. The Hot-TaqS DNA polymerase included in the set possesses a 5’ → 3’ polymerase- as well as a 5’-flap endonuclease activity, and generates a 3’dA (adenine)-overhang which may well be used for TA-cloning purposes.

Filled in colour coded tubes, the set contains the DNA polymerase and two 10x concentrated reaction buffers with 20 mM MgCl₂, one of which has been specially designed for direct gel loading following the PCR reaction. In 1 % agarose gels, the included red dye migrates approx. as fast as a 1 kb DNA fragment. During denaturation in Southern blotting, the dye turns yellow at an acidic pH. The use of the colourless PCR reaction buffer is adequate for all general PCR applications and is particularly recommended when direct fluorescence or absorbance readings are required.

Hot-TaqS polymerase in storage buffer containing 50 % glycerol, PCR buffer (10x) incl. 20 mM MgCl₂, PCR buffer red (10x) with 20 mM MgCl₂ and 0,1 % cresol red. Colour coded tubes.Contents of this set may not be bought separately.