Recombinant Human SERPINB9/PI-9 Protein (Human Cells, His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033318-10, PKSH033318-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: SERPINB9; PI-9

Target Symptom: Cytoplasmic antiproteinase 3; Peptidase inhibitor 9; CAP3; PI-9; Serpin B9

Research Area: Cell biology; immunology

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-6His

UNIProt ID: P50453

Accession: P50453

Background: Serpin B9; also known as Cytoplasmic antiproteinase 3(CAP-3); is a cytoplasm protein which belongs to the large superfamily of serine proteinase inhibitors (serpins); which bind to and inactivate serine proteinases. Serpin B9 is an inhibitor of the granzyme B/perforin lytic pathway. It is expressed in normal mammary epithelial cells but not in most mammary carcinoma cell lines. These interactions are involved in many cellular processes; including coagulation; fibrinolysis; complement fixation; matrix remodeling; and apoptosis. Serpin-B9 expression in immune-privileged cells; APCs; and CTLs protects these cells against the actions of granzyme B; and when expressed in tumor cells or virally infected hepatocytes; confers resistance to killing by CTL and NK cells. Expression of increasing levels of Serpin-B9 in target cells may progressively inhibit immune surveillance by blocking NK and CTL-induced cytotoxicity through the perforin / granzyme pathway and then through the Fas / FasL pathway.

Sequence: Met 1-Pro376

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB; 150mM NaCl; pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 43.4 kDa

Observed MW: 35-40 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only