Recombinant Mouse Carbonic Anhydrase 14/Car14 Protein (His Tag)(Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSM040972-10, PKSM040972-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Carbonic Anhydrase 14; Car14

Target Symptom: Carbonic Anhydrase 14; Carbonate Dehydratase XIV; Carbonic Anhydrase XIV; CA-XIV; CA14;

Target Species: Mouse

Expression Host: HEK293 Cells

Application: Enzyme

Fusion Tag: C-6His

UNIProt ID: Q9WVT6

Accession: Q9WVT6

Background: Mouse Ca14, also known as Carbonic anhydrase 14, is a member of large family of zinc metalloenzymes .It could catalyze reversible hydration of carbon dioxide. The reaction is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Fifteen CA isoforms have been reported so far. They have different patterns of tissue-specific expression and physiologic roles. Some CAs may serve as markers for tumors and hypoxia. CA XIV is a polypeptide consisting of an extracellular N-terminal catalytic domain, a membrane-spanning segment and a short intracellular C- terminal segment with several potential phosphorylation sites. A subset of CAs lack CA activity due to point mutations but retain esterase function. CA14 is widely expressed in the central nervous system

Activity: Measured by its esterase activity. The specific activity is 6556. 875 pmol/min/ug, as measured under the described conditions.

Sequence: Ala16-Met290

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 31.8 kDa

Observed MW: 41-50 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only