Recombinant Human IL10-RA/IL-10 Rα Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033632-10, PKSH033632-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: IL10-RA; IL-10 Rα

Target Symptom: Interleukin-10 receptor subunit alpha; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; CDw210a; Interleukin-10 receptor subunit 1; IL-10R subunit 1; IL-10R1; CD210; IL10RA; IL-10RA

Research Area: immunology

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-His

UNIProt ID: Q13651

Accession: Q13651

Background: Interleukin-10 Receptor alpha (IL-10Rα) is a transmembrane glycoprotein member of the class II cytokine receptor family. Mature human IL-10 Rα consists of a 214 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain. Within the ECD, human IL-10 Rα shares 59% aa sequence identity with mouse and rat IL-10Rα. IL-10 Rα is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer. IL-10Rα is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL-10 Rα, resulting in the recruitment of IL-10 Rβ. Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection. Polymorphisms of human IL-10 Rα may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL10.

Sequence: His22-Asn235

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 25.2 kDa

Observed MW: 38-59 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only