RBX1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91163-50, BS91163-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P62877(Human) P62878(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC IHC FC

All Applications: WB:1:500
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: Rbx1 (also designated ROC1 and Hrt1) and the closely related protein Rbx2 (also designated ROC2) are RING finger containing homologs of the yeast protein APC11, a member of the anaphase-promoting complex (APC). Rbx1 was shown to be a component of the von Hippel-Lindau (VHL) transcription elongation complex, which includes VHL, Elongin B, Elongin C and Cullin-2. Rbx1 interacts with Cullin-1 in the SCF (Skp1-Cdc53-F-box protein) ubiquitin ligase complex (2-4). Rbx1 functions as a common subunit of SCF complexes required for ubiquination of various proteins including yeast G1 cyclins, IkBa and b-catenin (2-4). Rbx1 was shown to enhance the ubiquitin ligase activity of the VHL/Cullin-2 complex, and of the SCF/Cullin-1 complex.

Purification and Purity: Protein affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: RBX1 polyclonal antibody detects endogenous levels of RBX1 protein.

Bioworld Molecular Weight: 12 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of RBX1 on HepG2 cell lysates using anti-RBX1 antibody at 1/500 dilution., ICC staining RBX1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Rbx1, RBX1, RNF75, ROC1, BA554C12.1, E3 ubiquitin-protein ligase RBX1, FLJ60363, MGC13357, MGC1481, OTTHUMP00000028983, Protein ZYP, Rbx 1, Rbx1, RBX1, Regulator of cullins 1, Ring box 1, Ring box 1 E3 ubiquitin protein ligase, RING box protein 1, RING finger protein 75, RING finger protein, RING-box protein 1, Ringbox protein 1, RNF 75, RNF75, ROC 1, ZYP protein,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified