CRYGC Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71460-50, BS71460-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07315

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:1000 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the beta/gamma-crystallin family of proteins. Crystallins constitute the major proteins of vertebrate eye lens and maintain the transparency and refractive index of the lens. This gene and several family members are present in a gene cluster on chromosome 2. Mutations in this gene have been shown to cause multiple types of cataract, including Coppock-like cataract and zonular pulverulent cataract, among others.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 17kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using CRYGC antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunofluorescence analysis of L929 cells using CRYGC antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of C6 cells using CRYGC antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: CRYGC; CCL; CRYG3; CTRCT2

Immunogen: Recombinant fusion protein of human CRYGC(NP_066269.1).

Conjugate: Unconjugated

Modification: Unmodified