Orai1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90988-50, BS90988-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96D31(Human) Q8BWG9(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Orai1 (ORAI calcium release-activated calcium modulator 1), also known as ORAT1, CRACM1 (calcium release-activated calcium modulator 1) or TMEM142A (transmembrane protein 142A), is a 301 amino acid multi-pass membrane protein that belongs to the Orai family of proteins. Localizing to the plasma membrane, Orai1 plays an important role in store-operated calcium (SOC) entry, a process involving Ca2+ influx and replenishment of Ca2+ stores formerly emptied through the action of inositol 1,4,5-trisphosphate production and other Ca2+ mobilizing agents. Specifically, Orai1 functions as a pore subunit of the store-operated calcium release-activated calcium channel (CRAC) and is essential for proper function of the CRAC channel. CRAC channels are responsible for mediating calcium influx in T-cells and play an important role in the immune response. Mutations in the gene encoding Orai1 can result in severe combined immunodeficiency (SCID).

Purification and Purity: Peptide affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Orai1 polyclonal antibody detects endogenous levels of Orai1 protein.

Bioworld Molecular Weight: 33 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Orai1 on PC-3M cell lysate using anti-Orai1 antibody at 1/500 dilution., ICC staining Orai1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: ORAI1, CRACM1, TMEM142A, Calcium release activated calcium channel protein 1, Calcium release activated calcium modulator 1, Calcium release-activated calcium channel protein 1, CRACM 1, CRACM1, CRCM1, CRCM1, FLJ14466, Orai 1, ORAI calcium release activated calcium modulator 1, orai1, ORAT1, Protein orai 1, Protein orai-1, TMEM 142A, TMEM142A, Transmembrane protein 142A,

Immunogen: Synthetic peptide within mouse Orai1 aa 150-250.

Conjugate: Unconjugated

Modification: Unmodified