Recombinant Mouse CD64/FCGR1 Protein (His&AVI Tag), Biotinylated(Active)

Sizes: 20μg, 100μg

Catalogue Numbers: PKSM040877-20, PKSM040877-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: CD64; FCGR1

Target Symptom: AI323638; AV092959; CD64; FcgammaRI; IGGHAFC

Conjugation: Biotinylated

Target Species: Mouse

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-His-AVI

Accession: NP_034316.1

Background: High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

Activity: 1. Immobilized Rituximab (IgG1) at 1 μg/mL can bind Biotinylated Mouse CD64 recombinant protein, the EC50 is 20-50 ng/mL. 2. Labeling ratio of biotin to protein is 0.7-1 by the HABA assay.

Sequence: Met 1-Pro 297

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 34.3 kDa

Observed MW: 50-55 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only