Securin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91218-50, BS91218-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95997(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IHC FC

All Applications: WB:1:500
IHC:1:200-1:500
ICC:1:200-1:500
FC:1:50-1:100

Background: The product of the oncogene PTTG, pituitary tumor transforming gene, is a human homolog of the anaphase-inhibitor vertebrate protein, securin. PTTG contains a basic amino-terminal domain and an acidic carboxy-terminal domain, which acts as a transactivation domain when fused to a heterologous DNA binding domain. Human PTTG is overexpressed in Jurkat and is also detected in human thymus, testis and placenta. PTTG is mainly expressed in the cytoplasm and is also partially localized to the nucleus. Vertebrate PTTG regulates the separin Esp1, which promotes chromatid separation, to overcome the cohesive forces that hold sister chromatids together. This regulatory function of PTTG suggests that defective regulation of cohesion may contribute to cancer by promoting chromosome instability. Although vertebrate PTTG shares cell-cycle functions with its yeast securin counterparts Pds1p and Cut2, none share sequence homology.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Securin polyclonal antibody detects endogenous levels of Securin protein.

Bioworld Molecular Weight: 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Securin on HUVEC cell lysate using anti-Securin antibody at 1/500 dilution., ICC staining Securin in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Tumor-transforming protein 1, hPTTG, PTTG1, EAP1, PTTG, TUTR1, AW555095, C87862, Cut2, EAP 1, EAP1, ESP1 associated protein 1, Esp1-associated protein, hPTTG, MGC126883, MGC138276, Pds1, Pituitary tumor transforming 1, Pituitary tumor transforming protein 1, Pituitary tumor-transforming 1, isoform CRA_a, Pituitary tumor-transforming 1, isoform CRA_b, Pituitary tumor-transforming gene 1, Pituitary tumor-transforming gene 1 protein, PTTG 1, PTTG, PTTG1, PTTG1 protein, PTTG1, Pttg3, Securin, Tumor transforming 1, Tumor transforming protein 1, Tumor-transforming protein 1, TUTR 1, TUTR1,

Immunogen: Recombinant protein with human Securin aa 1-200.

Conjugate: Unconjugated

Modification: Unmodified