PICK1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91074-50, BS91074-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NRD5(Human)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP, , FC

All Applications: WB:1:2,000-1:5,000
IP:1:10-1:50
FC:1:50-1:100

Background: Protein interacting with C kinase 1 (PICK1) is a PDZ-domain containing protein that is located in the perinuclear region and is phosphorylated in response to PKCα activation. PKCα, which is essential for the regulation of proliferation and differentiation in numerous cell types, contains within its catalytic region a PDZ-binding domain that is absent from other PKC isoforms. Mutation of the PICK1 PDZ domain inhibits the binding of PICK1 to PKCα. PICK1 also interacts with the carboxy terminus of a-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor, a neurotransmitter receptor located at excitatory synapses, suggesting that PICK1 plays a role in the modulation of synaptic transmission by targeting and anchoring AMPA to specific synapses.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: PICK1 polyclonal antibody detects endogenous levels of PICK1 protein.

Bioworld Molecular Weight: 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PICK1 on SK-Br-3 cell lysate using anti-PICK1 antibody at 1/5,000 dilution., Flow cytometric analysis of 293T cells with PICK1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Alternative Name: PICK1, PRKCABP, dJ1039K5, MGC15204, OTTHUMP00000028509, PICK 1, Pick1, PICK1, PRKCA binding protein, PRKCA-binding protein, PRKCABP, Protein interacting with C kinase 1, Protein interacting with PRKCA, Protein interacting with PRKCA 1, Protein kinase C alpha binding protein, Protein kinase C-alpha-binding protein, Protein that interacts with C kinase 1,

Immunogen: Recombinant protein within N-terminal human PICK1.

Conjugate: Unconjugated

Modification: Unmodified