Recombinant Human TMED1 (C-Fc)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033904-10, PKSH033904-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: TMED1

Target Symptom: IL1RL1-Binding Protein; Il1rl1l; IL1RL1LG; IL-1RL1LG; IL1RL1LGIL1RL1-binding protein; ST2L; T1/ST2 receptor binding protein; TMED1; Tp24

Research Area: Cell biology

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-Fc

UNIProt ID: Q13445

Accession: Q13445

Background: TMED1 (Transmembrane Emp24 domain-containing protein 1) is a member of the TMED family of proteins (gene name TMED1). The TMED family of proteins are localized to membranes of the early secretory pathway, including the endoplasmic reticulum and Golgi, and function in vesicular protein trafficking. TMED1 is a 59 kDa monomer and has been reported to exist as homodimer. It contains 1 GOLD domain and is widely expressed. TMED1 is important in regulating innate immune signaling through its interaction with ST2L. Specifically, the GOLD domain in TMED1 interacts with the TIR domain of ST2L, a receptor for IL 33. This interaction promotes ST2L association with IL-33, allowing downstream signaling cascade activating MAP kinases, p38, and JNK. Studies have shown knockdown of TMED-1 in HUVECs impairs the IL-33 induced response resulting in reduction of IL-6 and IL-8 productions.

Sequence: Ala24-Asn194

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 46.3 kDa

Observed MW: 55-65 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only