Recombinant Human LFA-3/CD58 Protein (Fc Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033594-10, PKSH033594-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: LFA-3; CD58

Target Symptom: Lymphocyte Function-Associated Antigen 3; Surface Glycoprotein LFA-3; CD58; LFA3; Ag3; CD58 antigen

Research Area: immunology

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-Fc

UNIProt ID: P19256

Accession: P19256

Background: Lymphocyte function-associated antigen 3 (LFA-3/CD58) is a single-pass type I membrane protein. CD58 is widely expressed on hematopoietic and non-hematopoietic human tissue and has been found on leukocytes; erythrocytes; endothelial cells; epithelial cells and fibroblasts of human origin. It is a Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells; antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition; the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.

Sequence: Phe29-Arg215

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 .
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 48.6 kDa

Observed MW: 35-81 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only