Recombinant Human Factor D Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033442-10, PKSH033442-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Factor D

Target Symptom: Complement factor D; CFD; Adipsin; C3 convertase activator; Properdin factor D; DF; PFD; ADIPSIN; ADN; DF; FACTOR D

Research Area: immunology

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-10His

UNIProt ID: P00746

Accession: P00746

Background: Complement factor D; also known as adipsin; is a member of the chymotrypsin family of serine proteases; which plays an essential role in host defense as the rate-limiting enzyme in the alternative pathway of complement activation. Complement factor D activates a convertase (C3bBb) responsible for cleavage of the complement protein C3; which leads to the activation of terminal complement component C5-9 to form the membrane attack complex on microbial or cellular surfaces. It also functions in the regulation of systemic energy balance and physiologic and pathologic processes; including immunity and inflammation.

Sequence: Ile26-Ala253

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 25.8 kDa

Observed MW: 28 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only